Optimization of excitation and emission wavelengths for the UHPLC fluorescence detector for priority polycyclic aromatic hydrocarbons (PAHs)

Abstract Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants (POPs) that widely distributed in the environment and cause significant environmental damage. Furthermore, they endanger human health by polluting food from natural processing. Therefore, it is necessary to accurately detect PAHs various sample matrices, which requires precise, practical, rapid detection methods. The purpose of this research develop a high sensitivity analysis method analyzing optimum excitation emission wavelengths EPA's 15 priority polyaromatic UHPLC fluorescence detector (Acenaphthene, Anthracene, Benzo[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenzo[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, Naphthalene, Phenanthrene, Pyrene). An average 17–25 analyses were performed for each hydrocarbon, optimized obtained. LOD levels between 2 90 ppt obtained with created direction. It worth mentioning limits achieved some PAH parameters lower than those reported literature after pre-concentration steps.